LOCUS       40924_2259        7641 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector nvYFP w-, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Le T, Liang Z, Patel H, Yu MH, Sivasubramaniam G, Slovitt M, 
            Tanentzapf G, Mohanty N, Paul SM, Wu VM, Beitel GJ.
  TITLE     A new family of Drosophila balancer chromosomes with a w- dfd-GMR 
            yellow fluorescent protein marker
  JOURNAL   Genetics 174 (4), 2255-2257 (2006)
  PUBMED    17057238
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Le T, Liang Z, Beitel GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2006) Biochemistry, Molecular Biology and Cell 
            Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 
            60208, USA
REFERENCE   3  (bases 1 to 7641)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7641)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2006"; volume: "174"; issue: "4"; pages: "2255-2257"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-2006) Biochemistry, Molecular Biology and Cell Biology, 
            Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      79..2813
                     /label=dfd HZ2.7 promoter
                     /note="dfd HZ2.7 promoter"
                     /regulatory_class="promoter"
     regulatory      2819..2999
                     /label=GMR enhancer
                     /note="GMR enhancer"
                     /regulatory_class="enhancer"
     CDS             3204..3920
                     /label=mVenus
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     polyA_signal    4196..4317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4882..5470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5644..6501)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6502..6606)
                     /label=AmpR promoter
     misc_feature    complement(7409..7641)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 aagcttggct gcaggtagca tgctgcagca gatctggtct agagcccggg cgaattcgcc
       61 ggcgcgccgg taccccgcgg ccgctctaga gaggaccggt ccgaaagtcg cgtcgtgaac
      121 atatgcagtc aattaaataa acatttatta accgagccac tgcacaaaag ctgttaacaa
      181 agccatttgg ccatttcaga aaatgaaaac gttctcaaaa ggtgttcgtg agttagttgt
      241 ggaagggggg tgtcgagggg ccagcaactg cgaccgagag tgtctctgtg tctctggacc
      301 tgaaaaaacg gtaattgatg gtcttggcca ggacggcgag tgcagttgtt gctttcgacc
      361 agcatggagc ccacatgcat ttgcagtcgc cctctctctt tctgggcgta tcgtcccact
      421 cctgactgga tttcacgcat ggaactcggt cggcgtcccg tctggcttgc tttacactat
      481 gagaatagtt tataatatcc ggttaaaaag tgtatataaa gaactcagat gcacaacgat
      541 aagcaaatta tattgctaaa aaaaatatat ataaaacaaa atgtattcat ataacattta
      601 gtatagatat cgctaattaa tcttaactat tagaaaattt ctttttattt ccctttttca
      661 atatgtctcc aatgaggact cttcattcaa tcttattctt caagtgtgga ttcggttttt
      721 tcggcgagtc ccaggttcca atccctcccg ggccatatac atagtaaatt cgaattgaat
      781 tttggcggcg cgttcattaa tcattatttc aatcatgaaa caaatgtgct cgcttggtgc
      841 tgatgcttgt tgttgttgtt cccattgcgt tgtatcttgt gaaattttgt tggtgttctg
      901 ccagccccgc atccataatc ccaaacccaa ccaccatccg aaatccccat ccccatcccc
      961 agctcctcct tttcgttggc catcttcgat gcgcttcaaa gattgataat ctctgggata
     1021 tctttaaatt taattagtca cattcataac agaccgccag gcagcgagcc gtaccaaatt
     1081 acatgttaca gcaaactgag cctcgctttt agccatgttt atgctcctcc ccttttccat
     1141 cagattttac atgatgctcc cgtcccgcgg cttgatatgt gtgagaaact gaattctccg
     1201 gtctatggtc tccggtttct tctccggagc tgccgttcga tatttccctg gccctttaat
     1261 gaaaaatcgc cgttgttact aattatgatt gacacagacg taattggaaa tttgagcaaa
     1321 gcttgtcgac cctaccgctt ccatctgagc cacttctggc atttcggctt cgacttcggc
     1381 agtcttcaaa aagtttcact aaattaaatg tttttagctc aaactattcg aaaagaaaaa
     1441 ctttctttga tggataagcg caagttttga ggggatttat caagcatggt gtgaaaaata
     1501 tttaaagaaa gttaagtcaa ttcctggacg gtataattgg gtcactcgat aaaaactcgt
     1561 agtgtaagat ataacttatc gaattttcta ggtcttttaa cttttaactg aaatttattt
     1621 gaaataaata tattgtagat taataacgca acgaaaaata atcttaatgt ctagtatact
     1681 agtatactag acattaatat atttaactta agctaaacaa agctgaatta ttgtaagggc
     1741 ttgacaaaaa gtatatacca tatgtacttt aagaacacta agtgttccat ttgttctatc
     1801 gttaagtatg tttgtccgct aagtatttat aaatttctca ttgcaaaaat atgatagata
     1861 acatttttta caacttacca aatgatttaa tcaaattaat tttctcaatc aatcctatca
     1921 ttagatgatt tttattttcg ttttgttgaa attttattac agtcattcac aaaattatgg
     1981 ataattgcgg cgagcttata caagatgagg ttcttgaaaa caattatcca tttattgtgg
     2041 ctaatttgat gacttaatta cggcattcgg aatgacaaat ttctagtgat atgttaatac
     2101 tttagtggca cctttaagtt attcgatcta aagggcatct tcccagagat cttctcactt
     2161 tcttgcgaga actcgaagac atggttttgc tacaaaaatg cgtaaacagc caacatcatt
     2221 aagcttaatt ttgaggagat ttcttaagtt tttggagcta atgcgtggca attaggggct
     2281 catttcttcc aaccgactgg cggcaaaaag cgatcgatgg ttcgctttta gcccgaagct
     2341 tttaaatggt ctacatgcaa catatatcgc cgcgatattc cgagtcccca tcggaggctt
     2401 cattatggtg agttgtgcag cgaaattaaa tcaatcagag ataatctgtc atggtaatgg
     2461 gtgcctgtcc agttccagta tcagcttttt tgtttaggcc ccaaagcgat cgagttggac
     2521 tcatttaagt gattcgtgta attattttaa gcagccgcag tgtaaatttc gttaaacatt
     2581 catctccgct tagacttgga gttcatctag gcccgatttg ggcataatat gacaggtaaa
     2641 cagttggaac cggtgataaa tagtctaaga tattttgaac cacatttaat tgtttcgggt
     2701 ctctgggaag gagggaaggt gactgcaaaa ctttcgattg gtattgtaaa tatgacttta
     2761 atgactataa aagtcaaaac tggctttaat tctgagagac acacactcta gtggatcctc
     2821 tagagtcgac ttaaaggcat ttcaagggtt tccactgggc tcgacttaaa ggcatttcaa
     2881 gggtttccac tgggctcgac ttaaaggcat ttcaagggtt tccactgggc tcgacttaaa
     2941 ggcatttcaa gggtttccac tgggctcgac ttaaaggcat ttcaagggtt tccactgggc
     3001 tcgaggagcg ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa
     3061 acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac
     3121 aagctaaaca atctgcagcc aagctgatcc tctagggtac gcgctagagt cgagaggcct
     3181 gtttaaacga tccaccggtc aacatggtga gcaagggcga ggagctgttc accggcgtgg
     3241 tgcccatcct ggtcgagctg gacggcgacg tgaacggcca caagttcagc gtgtccggcg
     3301 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     3361 agctgcccgt gccctggccc accctggtga ccaccctggg ctacggcctg cagtgcttcg
     3421 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3481 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3541 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3601 aggacggcaa catcctgggc cacaagctgg agtacaacta caacagccac aacgtgtata
     3661 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     3721 aggacggcgg cgtgcagctg gccgaccact accagcagaa cacccccatc ggcgacggcc
     3781 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca
     3841 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggc atcactctgg
     3901 gcatggacga gctgtacaag gcctccaagg cccccagccg ccaccgccga catcgccagc
     3961 gctcccgtag ccgcaatcgc agtcgaagcc gcagctccga gcgaaagcgc cgtcagcggt
     4021 cccgaagccg ctccagcgag cgacgccgct agtaagcgat cgcctgatca taatcagcca
     4081 taccacatgg cgcgccttgt agaggtttta cttgctttaa ggccggccaa aacctcccac
     4141 acctccccct gaacctgaaa cataaaatga atgttaatta acaattgttg ttgttaactt
     4201 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4261 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttacta
     4321 gtgcaggtgc agccttccac tgcgaatcat taaagtgggt atcacaaatt tgggagtttt
     4381 caccaaggct gcacccaagg ctctgctccc acaattttct cttaatagca cacttcggca
     4441 cgtgaattaa ttttactcca gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt
     4501 tttattccac gtaagggtta atgttttcaa aaaaaaattc gtccgcacac aacctttcct
     4561 ctcaacaagc aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc ggctatcgac
     4621 gggaccacct tatgttattt catcatgggc cagacccacg tagtccagcg gcagatcggc
     4681 ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga
     4741 cgatgtgcag ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc
     4801 gttggcgtaa cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc
     4861 cgagttcaag aagaaggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5041 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     5101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5281 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     5341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5761 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5941 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     6121 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     6181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6601 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     6661 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     6721 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     6781 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     6841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     6901 accgcaccga atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga
     6961 atgtgttgcg gagagcgggt gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg
     7021 gaggtgaatt atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa atatatcccg
     7081 gtgttttatg tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg taagtctttt
     7141 ggttagaaga caaatccaaa gtctacttgt ggggatgttc gaaggggaaa tacttgtatt
     7201 ctataggtca tatcttgttt ttattggcac aaatataatt acattagctt tttgaggggg
     7261 caataaacag taaacacgat ggtaataatg gtaaaaaaaa aaaacaagca gttatttcgg
     7321 atatatgtcg gctactcctt gcgtcgggcc cgaagtctta gagccagata tgcgagcacc
     7381 cggaagctca cgatgagaat ggccagacca tgatgaaata acataaggtg gtcccgtcgg
     7441 caagagacat ccacttaacg tatgcttgca ataagtgcga gtgaaaggaa tagtattctg
     7501 agtgtcgtat tgagtctgag tgagacagcg atatgattgt tgattaaccc ttagcatgtc
     7561 cgtggggttt gaattaactc ataatattaa ttagacgaaa ttatttttaa agttttattt
     7621 ttaataattt gcgagtacgc a
//
